World Journal of
Pharmaceutical and Life Sciences

OPTIMIZATION STUDIES ON THE PRODUCTION AND ACTIVITY OF XYLANASE OBTAINED FROM PAENIBACILLUS LAUTUS ISOLATED FROM MANGROVE SOIL

Rohini Prakash and Aruna K.*

ABSTRACT

Xylanases (endo-1,4-xylanase, EC 3.2.1.8) are hydrolytic enzymes which cleaves the ?-1,4- backbone of the complex plant cell wall polysaccharide, xylan which in turn releases xylooligosaccharides and xylose. The screening for xylanase producing bacteria from mangrove soil was carried out on M-9 medium containing 0.5% xylan followed by congo red test using the same medium. The xylanase production was detected by formation of zone of hydrolysis by the xylanase producing organism. Three xylanase producing isolates were obtained. The maximum xylanase producing bacterium was identified as Paenibacillus lautus on the basis of morphological, cultural, biochemical and 16S rRNA sequence analysis. Maximum production of xylanase was obtained by the isolate in a medium inoculated with 5% (v/v) of inoculum adjusted to 0.20 O.D. at 540nm containing peptone (10g/L), meat extract (3g/L), NaCl (5g/L) and beechwood xylan (5g/L), pH 7 incubated on a shaker (120 rpm) at 40°C for 48hrs. Maximum xylanase activity was observed at 50°C and pH 7. Metal ions like Ca+2, Mg+2, Mn+2, Cu+2, Fe+2 and Zn+2 were observed to inhibit enzyme activity. Inhibitors such as SDS, ?- mercaptoethanol, Tween 80 and EDTA were seen to inhibit the enzyme activity. The potential action of xylanase as detergent additive was also studied.

[Full Text Article]   [Download Certificate]