Abstract
HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND ATENOLOL AND PHARMACEUTICAL DOSAGE FORM
Dr. G. Pratap Kumar* and Naga Rani Undrakunta
ABSTRACT
A Simple, specific and sensitive an isocratic Estimation by RP-HPLC analytical Method were developed and validated for the quantification Amlodipine. And Atenolol Quantification was achieved by using the mobile phase (55 volumes of mixed phosphate buffers ph-4.0: 45 volumes of acetonitrate sonicate for 10 mins for removing gases ). Inertsil BDS C18 250×4.6mm ID, 5μm Particle size was used as stationary phase. The flow rate was 1.0 ml/min. Measurements were made at a isobestic wavelength of 220nm. The average retention times for Atenolol and Amlodipine was found to be 6.060 & 2.593 min. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear Atenolol and Amlodipine from 60-140μg/ml & 6-14 μg/ml for respectively. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Atenolol and Amlodipine.
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