World Journal of
Pharmaceutical and Life Sciences

SUBSTITUTION OF 6-BENZYLAMINOPURINE AND NAPHTHALENE ACETIC ACID BY LEAF EXTRACTS OF SYMPHYTUM OFFICINALE AND LANTANA CAMARA ON THE IN VITRO CULTURE OF BIOPHYTUM SP (OXALIDACEAE) AND PSOROSPERMUM NERVOSUM (CLUSIACEAE)

*Ravoniarison Nivohanintsoa Elinorovololona and Ravoniarinjatovo Miary Angelina

ABSTRACT

To replace the plant growth regulators NAA and BAP on the in vitro culture of Biophytum sp and Psorospermum nervosum, the effects of different concentrations (0.1 to 2.5%) of leaves extracts of Symphytum officinale and Lantana camara were studied and compared with 0.1% of BAP and 0.1% of NAA. For Biophytum sp, the maximum of buds were observed with 1% of leaf extract of Symphytum officinale and with 2.5% for the extract of leaves of Lantana camara. In Psorospermum nervosum, buds were produced on medium with 2.5% of S. officinale extract. The maximum shoots elongation of Biophytum sp was obtained with 0.5% to 2% of S. officinale extract and 2.5% for L. camara extract. In Psorospermum nervosum, it was observed in the presence of 2.5% of the both extracts. Thus, leaves extracts can replace 0.1% of BAP on the sprouting and growth of these species. The best rooting of Biophytum sp was obtained with leaves extracts at 2.5% for S. officinale and 0.1% for L. camara. As for Psorospermum nervosum, the best production and elongation of roots were favored with the same concentration (2.5%) of these extracts of leaves. So the substitution of NAA at 0.1% by these leaves extracts is also possible.

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