World Journal of
Pharmaceutical and Life Sciences

INHIBITION OF GROWTH OF CALCIUM HYDROGEN PHOSPHATE DIHYDRATE CRYSTALS BY COUROUPITA GUIANENSIS LEAVES EXTRACT

Vijay Nirusimhan K., Beatrice Valdaris and Dr. Kesavan Manjula*

ABSTRACT

Objective: To investigate the inhibitory effect of methanol extract of Couroupita guianensis leaves on the growth of calcium hydrogen phosphate dihydrate (CHPD) crystals. Methods: Calcium hydrogen phosphate dihydrate (CHPD) crystals were grown by the single diffusion gel growth technique and the inhibitory effect of methanol extracts of Couroupita guianensis leaves on the growth of CHPD crystals has been studied. The grown crystals were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Powder X-Ray diffraction (XRD) methods for further confirmations. Results: With an increase in the concentration of methanol extract of Couroupita guianensis leaves, the weight of the formed crystals were gradually reduced from 2.35 g to 0.13 g in CHPD crystals, respectively. The crystals are harvested from the CHPD were characterized by Fourier Transform Infrared Spectroscopy (FTIR) to confirm the functional groups and Powder X-Ray Diffraction technique (XRD) analysis to confirm the crystalline phases of the CHPD and hydroxyapatite (HAP) crystals. Results obtained are indicated that Couroupita guianensis (leaves) has the potential to inhibit the formation of CHPD crystals. Conclusion: This study confirms that using methanol extract of leaves of Couroupita guianensis can promote the formation of hydroxyapatite (HAP) crystals and reduce the nucleation rate of CHPD crystals, a major component of calcium urinary stone.

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