World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

GC-MS ANALYSIS AND ANTIMICROBIAL ACTIVITY OF SUDANESE CASSIA FISTULA LINN (CAESALPINACEAE) FIXED OIL

Dr. Abdel Karim, M.*, Hana K. and Khalid, M. S.

ABSTRACT

Cassia fistula fixed seed oil was studied by GC-MS. The oil was also assessed for antimicrobial activity. Twenty five components were detected by GC-MS analysis. Main constituents are: 9, 12-Octadecadienoic acid methyl ester (24.46%); 9-Octadecenoic acid methyl ester (17.26%); Stigmast-7-en-3-ol (15.75%); Hexadecanoic acid methyl ester(13.55%) and Methyl stearate (7.75%). The antibacterial activity of the oil was evaluated via the diffusion assay against five standard human pathogens (Gram positive: Staphylococcus aureus and Bacillus subtilis; Gram negative: Escherichia coli and Pseudomonasa aeruginosa and the fungus Candida albicans). The oil was active against all test organisms in the range: 100 - 50mg/ml. On the basis of its promising antimicrobial activity, it seems that this oil is a lead for further opimization.

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