World Journal of
Pharmaceutical and Life Sciences

DETERMINATION OF LD100 AND LD50 OF CARMIOSINE INTRAPERITONEALLY AND ORALLY ADMINISTERED IN ALBINO RATS

*Elekima Ibioku, Nwachuku Edna Ogechi and Boisa Ndokiari

ABSTRACT

The determination of LD100 and LD50 of carmiosine administered intraperitoneally and orally was carried out. A total of 68 albino rats weighing approximately 150g were used. In the determination of LD100 intraperitoneally, 10 rats were used with varying doses of 0.0g/kg, 0.17g/kg, 0.50g/kg, 1.0g/kg, 1.53g/kg, 2.0g/kg, 2.5g/kg, 3.33g/kg, 4.17g/kg, and 5.0g/kg while 10 rats were also used in the determination of LD100 orally with doses of 0.0g/kg, 5.0g/kg, 10.0g/kg, 12.5g/kg, 17.5g/kg, 22.5g/kg, 25.0g/kg, 32.5g/kg, 37.5g/kg and 40.0g/kg. In the acute study, rats were randomly separated into six groups. Each group had a total of four rats. The intraperitoneally treated groups were designated Aci, Bci, Cci, Dci, Eci and Fci with doses of 0.0g/kg, 0.17g/kg, 0.50g/kg, 1.0g/kg, 1.53g/kg, and 2.0g/kg respectively while that of oral were designated Aoc, Boc, Coc, Doc, Eoc and Foc were given doses of 0.0g/kg, 5.0g/kg, 10.0g/kg, 12.5g/kg, 17.5g/kg and 22.5g/kg respectively after LD100 dtermination. The LD50 of carmiosine given intraperitoneally and orally was calculated using the arithmetic method of karber. The LD100 was found to be 2.0g/kg and 22.5g/kg for intraperitoneally and orally treated rats respectively while the LD50 were 1.25g/kg and 13.37g/kg for intraperitoneal and orally route of administration. The LD50 obtained suggests that carmiosine is slightly toxic and practically non-toxic substance when administered intraperitonelly and orally respectively. The discrepancies seen in the intraperitoneal and orally treated rats could be due to the difference in the route of administration.

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