Abstract
GC-MS ANALYSIS AND ANTIMICROBIAL ACTIVITY OF SUDANESE LUFFA AEGYPTIACA (LINN.) M.ROEM FIXED OIL
Prof. Abdel Karim. M.*, Hashim H. and Khalid M. S.
ABSTRACT
This study was planed to identify the constituents of Luffa egyptiaca fixed oil, which find many applications in ethnomedicine, and to evaluate its antimicrobial activity. Luffa egyptiaca oil was analyzed by GC-MS and identification of constituents was accomplished by comparison with the MS library (NIST) as well as the observed fragmentation pattern. The GC-MS analysis showed the presence of 17 components. Major constituents are : i) 9,12-octadecadienoic acid methyl ester(51.97%) , ii)9-Z-octadecenoic acid methyl ester(12.83%), iii) hexadecanoic acid methyl ester(11.84%), iv)methyl stearate(8.27%) , v)9,12-octadecadienoyl chloride(5.56%). The oil exhibited excellent activity against Pseudomonas aeruginosa and Bacillus subtilis in the range: 100-25mg/ml and 100-12.50mg/ml respectively. It also showed significant activity against Escherichia coli at 100-25mg/ml. At 100mg/ml, it exhibited significant activity against the yeast Candida albicans.
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