World Journal of
Pharmaceutical and Life Sciences

BIOSYHTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM LEAF EXTRACT OF INDIGOFERA HIRSUTA. L. AND IT’S ANTIMICROBIAL ACTIVITY

Dr. S. R. Siva Kumar*, R. Anitha and T.Sheela

ABSTRACT

In present study silver nanoparticles were synthesized from aqueous leaf extract of Indigofera hirsuta L. plant leaf extracts will be collected were used and compared for their extracellular of metallic silver nanoparticles. Stable silver nanoparticles formed to be treated aqueous by solution of AgNO3 with the plant leaf extracts as reducing agent Ag-to Ag+. We successfully characterized the biologically synthesized Ag-nanoparticles, which had an average size of 18-20 nm. These nanoparticles showed antibacterial activity against Pseudomoas aeruginosa and E. coli. UV spectroscopy is used to monitor the quantitative formation of silver nanoparticles. The synthesized silver nanoparticles are characterized with FT-IR, XRD, SEM and EDX Characterization by the above said instrument analysis confirmed the presence, size and stability of the silver nanoparticles. After characterization, the silver nanoparticles were tested at various concentrations to check their bactericidal activity against clinical isolates of five bacterial pathogens. The silver nanoparticles exhibited good bactericidal activity at all concentration against all the tested organisms. Maximum zone of inhibition was of observed against Vibrio cholera (18mm at 400g) and minimum level of antibacterial activity was observed against Proteus mirabilis (8mm at 100g). This result suggested the potential use of silver nanoparticles against other clinical pathogens.

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