World Journal of
Pharmaceutical and Life Sciences

PHYTOCHEMICAL ANALYSIS AND ISOLATION OF COMPOUNDS FROM METHANOLIC LEAF EXTRACT OF AMARANTHUS SPINOSUS AND ITS ANTIMICROBIAL EFFECT AGAINST STAPHYLOCOCCUS AUREUS

Jayashree Majumdar, Sangita Boro*, Bhriganka Bharadwaj and Balagopalan Unni

ABSTRACT

The crushed leaf of Amaranthus spinosus is used to treat skin infections and wound. The leaves are boiled without salt and consumed for 2-3 days to cure jaundice. Recent studied showed that Amaranthus leaves contain various health essential nutrients that exert anti-inflammatory action against skin infection. The aim of the study is to see the effect of isolated compounds from the methanolic leaf extract of Amaranthus spinosus against Staphylococcus aureus. In our study we found that the methanolic leaf extract of Amaranthus spinosus contained alkaloids, carbohydrate, glycosides, saponin, steroids and flavonoids. Antimicrobial activity of the crude extract showed an efficient activity against S. aureus. On performing the column chromatography of the leaf methanolic extract using hexane, mixture of hexane-methanol and methanol we obtained 17 fractions. The isolated compounds in Fraction 1 and Fraction 2 gave positive saponin test, Fraction 4 and 7 gave alkaloids positive test. Fraction 9, 12 and 15 showed the positive result of glycosides and steroids, Fraction 13 showed positive flavonoids test. Fraction 16 gave positive carbohydrate test. On performing the antimicrobial activity of these fractions against Staphylococcus aureus it was found that Fraction 4, 7, 12 and 15 showed antimicrobial activity against S. aureus whereas the rest of the fractions showed no activity at all.

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