World Journal of
Pharmaceutical and Life Sciences

ISOLATION AND IDENTIFICATION OF HELICOBACTER PYLORI FROM CULTIVATED AND COMMERCIALLY SOLD VEGETABLES AND SALAD FROM SOME SELECTED TOWNS (NNEWI, AND ODEKPE) IN ANAMBRA STATE

Obi M. C., Ochiabuto O. M. T. B., Odili F., Onwuasoanya U. F., Ekekwe E. N., Akulue T., Ekwunoh P. O., Chijioke O. U. and Obeagu E. I.*

ABSTRACT

Helicobacter pylori plays an important role in peptic ulcer disease and gastric carcinoma.This study was conducted to isolate and identify Helicobacter pylori from 64 cultivated and 60 commercially sold vegetables and salad materials in Odekpe and Nnewi towns in Anambra state Nigeria using conventional culture technique on selective Columbia blood agar base and antibiotic supplement(Oxoid, England). Identification was done using biochemical tests. Antibiotic susceptibility was also done with Kirby Bauer disc-diffusion method using Mueller Hinton agar (HiMedia Laboratories, Mumbai, India) according to the Clinical Laboratory Standards Institute.(CLSI 2012).Results revealed that a total of 6(9.4%) and2(3.3%) was isolated from vegetables from Odekpe and Salad materials from Nnewi towns with no significant difference (P>0.05;P0.171,X21.873). Pumpkin leaves was more contaminated in Odekpe 1(16.7%) and leek in Nnewi.2(100%).Unwashed samples recorded higher prevalence than washed, with no significant difference. (P>0.05;p0.144,x 2.138). Animals walking around farm, type of animal and source of water for irrigation showed marked significant association with the presence of organism. (P<0.05; P0.007, 0.009, 0.002: X27.30, 6.84, 15.28).Highest resistance to antibiotics was recorded in Nalidixic acid 8(100%) and Cephalothin 8(100%) and susceptibility to Chloramphenicol 8(100%) and Ciprofloxacin 8(100%). In conclusion, vegetables from Odekpe town were highly contaminated with H. pylori. Unwashed vegetables pose a threat to H. pylori contamination. Knowledge on type of animal walking around the farm, water source used for irrigation should be disseminated as a risk factor to H. pylori spread.

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