World Journal of
Pharmaceutical and Life Sciences

GENETIC VARIATION IN CITRUS CULTIVAR SIAM (CITRUS NOBILIS LOUR. VAR MICROCARPA HASSK) FROM GUNUANG OMEH PRODUCTION CENTRE, WEST SUMATERA, INDONESIA

 Melza Mulyani, Cici Catrina, Wiliardi, Dewi Imelda Roesma and Mansyurdin*

ABSTRACT

Fruit characteristics and genetic variation of has been conducted to know source variations of fruit quality product now and to support breeding program. Analysis genetic variation with Random Amplified Polymorphic DNA (RAPD) technique. The test result of 17 primers showed that three primers can amplified DNA Siam citrus of Gunuang Omeh are OPA-02, OPA-13 and OPB-10. Amplification result with three primers displayed 26 bands with average 8.6 bands per primer, OPA-02 showed at most with 11 bands. The proportion of polymorphic loci obtained 100%. Genetic variation value after seed intensification is higher than before intensification group with genetic diversity value (H= 0.3198) and Shannon Diversity Index (I= 0.4809). Heterozygosity value intra-subpopulation (Hs= 0.2874) higher than heterozygosity value inter-subpopulation (Dst= 0.0112) while differentiation value among two population is low (Gst= 0.0378).

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