World Journal of
Pharmaceutical and Life Sciences

PHYTOTOXICITY ASSESSMENT OF SECONDARY METABOLITE OF FUNGI ISOLATED FROM HYPTIS SUAVEOLENS. L

Firdous Ahmad Dar*, Farah Naseem, A. K. Pandey and Kirti Jain

ABSTRACT

During survey undertaken at R.D.V.V. campus, 4 fungal isolates were recovered from diseased parts of Hyptis suaveolens. Out of all the isolated strains, Nigrospora sp.FGCC#74 was found to be the most dominant pathogen with maximum herbicidal potential against Hyptis. Nigrospora is known to incite severe wilt, chlorosis and necrosis in Hyptis suaveolens. More or less all the strains recovered are known to synthesize phytotoxic metabolites. Primary and secondary screening test conducted to select potential strains revealed that Nigrospora sp. produces secondary metabolite with very high herbicidal activity against Hyptis suaveolens. Further, the toxic metabolite residing in Cell Free Culture Filtrate was extracted with carbon tetra chloride, chloroform, ethyl acetate, and butanol .The residue obtained after solvent evaporation were evaluated separately for there phytotoxicity against the Hyptis leaves by detached leaf bioassay .Residue obtained from butanol fraction exhibited maximum toxicity i.e. by showing chlorosis and necrosis when compared with others. This is the first report confirming the potential of this particular toxin from Nigrospora sp. as a biorational, which can be applied as phytotoxin against Hyptis suaveolens.

[Full Text Article]   [Download Certificate]