World Journal of
Pharmaceutical and Life Sciences

IDENTIFICATION ACTIVE COMPOUNDS OF BACTERIA STREPTOMYCES USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Dr. Mohsen Hashim Risan*, Bassam Qasim, Ban Ameen Abdel-jabbar and Athraa H. Muhsin

ABSTRACT

Aim of the study assess optimum Growth of Streptomyces and identification active compounds of bacteria Streptomyces using HPLC. The biochemical and physiological testes of Streptomyces sp. Show that the Streptomyces produced extra cellular enzymes like amylase, urease, catalase, lipase, protease, pectinase, cellulase and phosphatase, no HCN production and negative for indole production. Estimating the stationary phase for isolated Streptomyces were growing on malt extract yeast extract broth ( ISP2), and getting the optical density (OD) at 600nm during (1-7) days and optimal growth of them ranged (6.5 to 8), while were salt concentrations ranged from (1 to 7% NaCl). The best growth happened at 30 °C by measuring the absorbance of the growth and getting the optical density (OD) at 600nm. We watched the growth state activity of isolated strain which started after 72 hours of incubation at 30°C, when the OD was about (0.572 ). The HPLC analysis of extracellular crude extract was showed found five different antibiotic (Tetracycline, Streptomycin, Neomycin, Vancomycin and Kanamycin).

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