World Journal of
Pharmaceutical and Life Sciences

CTX-M TYPE EXTENDED SPECTRUM β-LACTAMASES IN ESCHERICHIA COLI ISOLATES FROM CLINICAL SAMPLES FROM KALABURAGI REGION

Jahanara Kudsi and Prof. Kelmani Chandrakanth R.*

ABSTRACT

The ?-lactam antibiotics, along with amino glycosides are widely prescribed antibiotics. However resistance to these drugs continues to increase due to their injudicious use. In recent years, resistance in the Indian E.coli population has increased and mostly showing complex mechanisms like extended spectrum ?-lactamases (ESBLs) specifically blaCTX-M. The reason may be increased intracontinental movement of the human population. So it should be better to know the prevalence and resistance pattern of these strains, well in advance, to design a systematic policy for empirical therapy. Methods: 150 isolates of Escherichia coli obtained from 170 non-repeat samples of urine, blood, stool, us etc were studied and their resistance rates and patterns were noted. The isolates were screened for prevalent cephalosporin’s resistance (ESBLs) by antibiotic susceptibility test. Minimum inhibitory concentration (MIC) for cephotaxime and ceftazidime was done by HiComb MIC-test strips ESBLs were confirmed by NCCLS-ESBL phenotypic confirmatory test (Combined disc diffusion test) and E-test. Test was carried out using cephotaxime (30?g), ceftazidime (30?g), cephotaxime/clavulanicacid (30/10?g) and ceftazidime/clavulanic acid (30/10 ?g) discs. Genotypic conformation for presence of ESBL (CTX-Type) was established by PCR. Results: Resistance to 5 beta-lactam antibiotics tested was varying between 70 to 80 percent i.e. approximately 120 isolates. MICs of ESBL producing isolates ranged from 8 to > 240 ?g/ml for both ceftazidime and cephotaxime. 110(92%) of above 120 resistant strains were positive for ESBL production both by the E -test for ESBL and ESBL detection kit (Combination disk method). The PCR band after amplification at 544 bp was visualized.

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