World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

METHOD DEVELOPMENT AND VALIDATION OF DRUG COMBINATION IN A FORMULATION OF ISONIAZID, RIFAMPICIN AND PIPERINE BY RP-HPLC METHOD

Merugu Manasa* and Mahesh Pothu

ABSTRACT

A simple, isocratic reverse phase high pressure liquid chromatographic method has been validation and method development of new drug combination. The method was carried out on an Waters xterra reverse phase C 18 (250 mm x 4.6 mm I.D; particle size 5 ?m) column with a mobile phase consisting of methanol and ortho phosphoric acid (pH was adjusted to 2.2 by using hydrochloric acid) in the ratio of 40:60 at a flow rate 1.0 mL/min. Detection was carried out at ? 282 nm. The retention times of Isoniazid , Rifampicin and Piperine were found to be 2.513, 3.625 and 5.316 min respectively. The developed method was validated in terms of accuracy, precision, limit of detection/quantitation, linearity and robustness. The proposed method is highly sensitive, precise, accurate and easy to perform and successfully applied for the estimation of these drugs in bulk and capsule dosage form.

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