World Journal of
Pharmaceutical and Life Sciences

MOLECULAR DETECTION OF MULTI DRUG RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX ALONG WITH IDENTIFICATION OF NON-TUBERCULOUS MYCOBACTERIUM SPECIES FROM SPUTUM SAMPLES IN A TERTIARY CARE HOSPITAL

Sana Nudrat*, Umar Farooq, Shivendra Mohan

ABSTRACT

Background: Tuberculosis is the second leading cause of death in developing countries. Multi-drug resistant tuberculosis (MDR) is a form of tuberculosis that is resistant to rifampicin and isoniazid. Extensively drug resistant (XDR) is resistant to isoniazid and rifampicin with resistant to one of the fluoroquinolones as well as resistant to at least one of the injectable drug of second line. Objectives: Detection of the MDR and XDR status and NTM identification from smear positive sputum samples by Molecular technique. Material and Methods: Study was performed on smear positive sputum samples collected from DOTS centre at TMMC and RC, Moradabad during period of July.15 to Dec.16. Samples were decontaminated by pettrof method and then DNA was extracted by using heating method. Then were subjected to PCR using Applied Biosystem Veriti 96 well Thermal Cycler and LPA by using GenoType MDRplus VER.2.0 kit on Twincubater of HAIN life sciences. Result: Total 170 smear positive sputum samples were processed by line probe assay, in which 14 were MDR, 2 were XDR and 10 were detected NTM. Conclusion: In this study, we concluded that line probe assay should be used on routine basis for drug sensitivity of M.tuberculosis cases, so that appropriate treatment can be given on time.

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