World Journal of
Pharmaceutical and Life Sciences

IDENTIFICATION OF CARBAPENAMASE PRODUCTION IN MULTI DRUG RESISTANT GRAM NEGATIVE BACTERIA BY PHENOTYPIC METHODS FROM A TERTIARY CARE HOSPITAL OF WESTERN U.P. INDIA

*Shivendra Mohan, Umar Farooq and Sana Nudrat

ABSTRACT

Objectives: To determine the occurrence of carbapenam resistance among MDR-GNB isolates by phenotypic methods. Introduction: Cabapenams posses the broadest spectrum of activity against gram positive and gram negative bacteria, and play a critically important role in our antibiotic armamentarium. Detection of carbapenamase enzyme production is a crucial infection control issue because they are often associated with antibiotic resistance, treatment failures and infection associated mortality. Material and methods: Study was performed during April 2016 to October 2016. Isolates found resistant to?3differnt groups of antibiotics were subjected to phenotypic test I.e.; Modified Hodge Test (MHT) and MBL E strip Test for detection of carbapenamase enzyme production. Results: Total 105 samples were processed by conventional culture method and antibiotic sensitivity test by Kirby Bauer disk diffusion method and 45 were found resistant to?3 different groups of antibiotics. Out of 45 resistant cases 09 were positive for MHT and 07 were MBL E Strip test positive. Conclusion: Simple, rapid and cost effective test are required to be done on a routine basis for detection of carbapenamase enzymes along with class determination so that appropriate antibiotic treatment can be started in cases of extensive antibiotic resistance.

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