World Journal of
Pharmaceutical and Life Sciences

PHYTOCHEMICAL SCREENING, IN-VITRO ANTIOXIDANT EFFICACY & ANTHELMINTIC ACTIVITY OF CHLOROFORM- METHANOLIC EXTRACT OF DALBERGIA SISSOO

Abinash Kumar Sahu*, Sweta Shankar Pal, Ritwik Dutta, Sushant Kumar, Farbish Sahu and Godti Yamini

ABSTRACT

The total objective of our study must be to identify drugs to estimate human illness by a thorough analysis of plant Ayurveda and modern medicine techniques must be coupled in order to bring out high quality of herbal product with rapid onset of action and good bioavailability. Aim: The aim of the study is to investigate Phytochemical screening of Chloroform-methanolic Extract of Dalbergia sissoo. Result & Conclusion: The qualitative phytochemical analysis of Chloroform:Methanol (60:40) extract & Aqueous extract of Dalbergia sissoo was done with preliminary identification of different bioactive compounds such as alkaloids, glycosides, Cardiac Glycosides, flavonoids, tannins, carbohydrate, steroids and saponins are present. The content of total phenolic compounds (TPC) was expressed as mg/g of gallic acid equivalent of dry extract sample using the equation obtained from the calibration curve. Total flavonoids content was calculated as quercetin equivalent (mg/g) using the equation based on the calibration curve: Y=0.110 X+0.004, R2=0.999, where X is the absorbance and Y is the quercetin equivalent (QE). The amount of total flavonoids was determined with the Quercetin. Quercetin was used as a standard compound and the total flavonoids were expressed as mg/g Quercetin equivalent using the standard curve equation: Y = 0.008X + 0.007, R2= 0.999, Where Y is absorbance at 420 nm and X is total flavonoids content in the extracts of Dalbergia sissoo expressed in mg/gm. DPPH scavenging activity has been used for screening the in vitro antioxidant activity of plant extracts. The absorption maximum of a stable DPPH radical in methanol was at 517nm in U.V spectrophotometer. IC50 for standard Ascorbic acid was found to be 59.69 μg/ml and for Dalbergia sissoo was found to be 185 μg/ml. Thus the anti-oxidant activity of sample was less than that of standard ascorbic acid. The data revealed for anthelmintic activity state that the aqueous extract has a better wormicidal effect than Chloroform: Methanolic extract with compared with the standard drug Albendazole. Further study is required to find out the novel phytoconstituents responsible for anthelmintic action against various helminthes.

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