World Journal of
Pharmaceutical and Life Sciences

FORMULATION AND EVALUATION OF ANTI FUNGAL CREAM BY USING PSIDIUM GUAJAVA

Deepti K. Salunke*, Akshada G. Waghchaure, Usha N. Jain, Jayshree V. Gavit and Sanket K. Raut

ABSTRACT

Fungal infections are a significant global health concern, ranging from superficial to systemic infections. Psidium guajava (Guava) leaf extract has demonstrated antifungal activity against a wide variety of fungi. This research focuses on the development and evaluation of a cream containing guava leaf extract. Ethanolic, methanolic, and hydroalcoholic extracts of the leaves were assessed for total flavonoid content, thin layer chromatography (TLC), high- performance thin layer chromatography (HPTLC), and antifungal activity against Candida albicans. The cream formulation was optimized using a 3² factorial design, with beeswax and Tween 80 selected as independent variables. Their effects on viscosity and cumulative drug diffusion were studied. The optimized cream was evaluated for its appearance, pH, washability, spreadability, in-vitro The hydroalcoholic extract exhibited the highest flavonoid content and showed a superior zone of inhibition (27 ± 1 mm) against Candida albicans. HPTLC confirmed the presence of key flavonoids. A cream containing 6% beeswax and 5% Tween 80, with a viscosity of 10,420 P and 70% drug diffusion, was identified as the optimal formulation. This cream met alevaluated criteria and displayed an antifungal zone of inhibition (25 mm), which was larger than the standard formulation (23 mm). No signs of skin irritation were observed in the study, making this formulation a promising alternative to existing topical antifungal treatments. drug diffusion, antifungal efficacy, and potential for skin irritation in Wistar rats.

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