World Journal of
Pharmaceutical and Life Sciences

ULTRAVOILET-VISIBLE SPECTROSCOPY: A REVIEW

Payal M. Bisen*, Divya M. Bisen, Rani T. Bhagat, Rashmi D. Tembhare, Mrunali Y. Rinayate and Tulsidas P. Nimbekar

ABSTRACT

Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beam of light after it passes through a sample or after reflection from a sample surface. This article uses the term UV-Vis spectroscopy to include a variety of absorption, transmittance, and reflectance measurements in the ultraviolet (UV). Visible and near-infrared (NIR) spectral regions. These measurements can be at a single wavelength or over an extended spectral range. This article provides an overview of the technique and does not attempt to provide a comprehensive review of the many applications of UV-Vis spectroscopy in materials research. In this regard, many of the references were chosen to illustrate the diversity of applications rather than to comprehensively survey the uses of UV-Vis spectroscopy. Rapid and easy analytical methods are needed due to increasing number of multicomponent formulation bio therapeutic product and sample of complex matrix Number of ultraviolet spectrophotometer method use for these purposes. Ultraviolet and visible absorption Spectroscopy is the measurements of the attenuation of a beam of light after reflection from a sample surface.

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