World Journal of
Pharmaceutical and Life Sciences

GLYPHOSATE AND ALUMINUM PHOSPHIDE INDUCED DNA OXIDATION AND INFLAMMATION IN ALBINO RAT: POTENTIAL CHRONIC CONDITION FOR CANCER DEVELOPMENT

Ezeani Ujuamala Uloma, Afonne Onyemaechi Johnson and Ezeani Michael Chukwudi*

ABSTRACT

Pesticides have been associated with increased risk of chronic disease such as cancer, yet their fundamental role that portends their association with such chronic disease is not yet well explored. This study evaluated the serum level of DNA oxidative and inflammatory markers in albino rats exposed to glyphosate and Aluminum phosphide. The chemicals were administered orally at intervals of one month up to third month, based on low and high dose assessment, to groups of male and female albino rats: Serum levels of 8-OHDG, IL-6 and IL-10 were determined using Enzyme Linked Immunosorbent Assay (ELISA). There was significant increase in mean level of 8-OHDG in male/female rats exposed to GLP on first, second and third exposures respectively (low dose P=0.052/0.052, P=0.037/0.026, P=0.742/0.011, respectively); (high dose. P=0.000/0.019, P=0.027/0.004, P=0.019/0.019 respectively). There was significant increase in mean level of IL-6 in male/female rats exposed to GLP on first, second and third exposures respectively (low dose P=0.011/0.008, P=0.007/0.002, P=0.000/0.000, respectively); (high dose. P=0.001/0.005, P=0.000/0.003, P=0.000/0.000 respectively). There was significant increase in mean level of IL-10 in male/female rats exposed to GLP on first, second and third exposures respectively (low dose P=0.802/0.254, P=0.008/0.003, P=0.000/0.004, respectively); (high dose. P=0.407/0.008, P=0.003/0.043, P=0.000/0.010 respectively). There was significant increase in mean level of 8-OHDG in male/female rats exposed to ALP on first, second and third exposures respectively (low dose P=0.025/0.026, P=0.026/0.009, P=0.025/0.021, respectively); (high dose: P=0.002/0.001, P=0.073 and 0.170 respectively). There was significant increase in mean level of IL-6 in male/female rats exposed to ALP on first, second and third exposures respectively (low dose P=0.016/0.007, P=0.026/0.005, P=0.000/0.000, respectively); (high dose: P=0.002/0.007, P=0.002/0.002 P=0.000/0.007 respectively). There was significant increase in mean level of IL-10 in male/female rats exposed to ALP on first, second and third exposures respectively (low dose P=0.154/0.182, P=0.001/0.007, P=0.000/0.000, respectively); (high dose: P=0.063/0.018, P=0.001/0.003 P=0.000/0.000 respectively). Glyphosate and aluminum phosphide mediated increase in the levels of DNA oxidative and inflammatory markers (8-OHDG, IL-6 and IL-10), thereby leading to deregulation of the immune system. It is also imperative that persistent exposure to these chemicals could sustain chronic inflammation and under this condition, such inflammatory cytokines as IL-6, could mediate chronic oxidative stress inducing persistent DNA damage. Exposure to GLYP and ALP could be a potent source of cellular oxidative stress and its persistence, mediating chronic inflammation, thus existing as underlying causal effects to many chronic diseases including Cancer.

[Full Text Article]   [Download Certificate]