World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

REVIEW ARTICLE ON EXPLORING CHROMATOGRAPHY: PRINCIPLES, TECHNIQUES & APPLICATION

Afroja A. Mujawar*, Soundrya P. Nikam, Dhairyasheel S. Chavan, Vinayak S. Mane, Mayuri Y. Choundikar

ABSTRACT

A separation method called chromatography separates a mixture into discrete molecules according to their solubility, affinity, and interaction with a solid phase. In a moving phase, constituents travel across a stationary phase at varying speeds. Thin layer chromatography method(TLC) and column chromatography method are two examples of the several kinds of chromatography. TLC uses adsorption and partition to separate materials on a plate; ninhydrin is frequently used for visualization. By passing a mobile phase through a solid stationary phase, column chromatography—including HPLC (High Performance Liquid Chromatography)—separates mixtures and enables component identification, measurement, or purification.

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