World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND ENCAPSULATION OF EXTRACTED SEED KERNEL OIL OF BUCHANANIA LANCEOLATA WIGHT

*Jisa Ann Sabu, S. Reshma and Dr. Brijithlal N. D.

ABSTRACT

Buchanania lanceolata Wight. is a tree species from Anacardiaceae family. It is classified as Vulnerable under IUCN Red List of Threatened Species. In Indian traditional medicine, B. lanceolata has various medicinal and therapeutic properties. The present study focuses on the extraction of oil from the seed kernels of B. lanceolata for testing its in vitro anti-inflammatory property followed by the encapsulation of oil in calcium alginate beads. The preliminary qualitative phytochemical analysis of the extracted seed kernel oil shows the presence of alkaloids, terpenoids, fatty acids and flavonoids. The total of 13 volatile bioactive compounds in the oil were identified by GC-MS analysis. The functional groups and bonds of the active phytoconstituents were detected by FTIR analysis. The in vitro anti-inflammatory activity, assessed by protein denaturation assay revealed that the sample as well as the reference medication, Diclofenac inhibited the protein denaturation in a concentration dependent manner throughout a range of 6.25 to 100 μl. The extract at the dose of 6.25μg/ml and 12.5μg/ml exhibited better anti- inflammatory activity with the percentage inhibition of 14.42 % and 22.39 % respectively. Essential oil requires encapsulation to prevent its degradation due to environmental factors. For increasing its shelf life, the oil was encapsulated in calcium alginate beads and stored for further analysis. The overall result demonstrated that the seed kernel oil of B.lanceolata have various bioactive compounds and exhibits good anti-inflammatory activity.

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