World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF PREGABLIN AND 4-ISOBUTYLPYRROLIDIN-2-ONE USING ANIMAL MODELS

Faheem Unnisa*, Dr. Syed Ahmed Hussain, Arshiya Tarannum, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima and Nazneen

ABSTRACT

Aim: To evaluate the cytotoxic effects of Lipoxal, an alternative platinum-based compound, on kidney cancer cell
lines using various viability assays and compare its efficacy with the standard chemotherapeutic agent Vinblastine.
Objective: The primary objective is to analyze and quantify cell viability after treatment with Lipoxal at different
concentrations and measure its impact using MTT, CellTiter-Glo, Alamar Blue, SRB, and LDH Cytotoxicity
assays. Research: Kidney cancer cells were treated with increasing concentrations of Lipoxal (1 μM, 5 μM, and 10
μM). Each assay was conducted to assess cell viability and cytotoxicity. The results demonstrated a dosedependent
decrease in cell viability, with a significant reduction observed at higher concentrations. Conclusion:
Lipoxal exhibited notable cytotoxic effects on kidney cancer cells, with a reduction in cell viability observed across
all assays. The findings suggest that Lipoxal could serve as a potential alternative to Vinblastine for the treatment
of transitional cell carcinoma (TCC), especially for patients resistant to cisplatin therapy.

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