World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF PREGABLIN AND 4-ISOBUTYLPYRROLIDIN-2-ONE USING ANIMAL MODELS

Faheem Unnisa*, Dr. Syed Ahmed Hussain, Arshiya Tarannum, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima and Nazneen

ABSTRACT

Aim: To evaluate the cytotoxicity and cell viability of Vinflunine ditartrate, a third-generation vinca alkaloid, in
comparison to the standard drug Vinblastine, for the treatment of transitional cell carcinoma (TCC). Objective: To
analyze the efficacy of Vinflunine ditartrate using various in vitro assays, including MTT, CellTiter-Glo, Alamar
Blue, SRB, and LDH, and determine its potential as an alternative therapeutic option. Research: Vinflunine
ditartrate was tested against kidney cancer cell lines using multiple assays to assess cell viability, metabolic
activity, and cytotoxicity. Each assay provided quantitative insights into the effect of various concentrations (1, 5,
and 10 μM) of Vinflunine ditartrate on cell survival. Results were compared to the control treatment with
Vinblastine to establish relative efficacy. Conclusion: Vinflunine ditartrate demonstrated a dose-dependent
reduction in cell viability and increased cytotoxicity across all assays. The compound showed promising anticancer
properties, indicating its potential role as a second-line therapeutic agent for TCC treatment.

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