World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF PREGABLIN AND 4-ISOBUTYLPYRROLIDIN-2-ONE USING ANIMAL MODELS

Dr. Syed Ahmed Hussain*, Faheem Unnisa*, Arshiya Tarannum, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima and Nazneen

ABSTRACT

Aim: The aim of this study was to evaluate the cytotoxic effects of Encequidar on kidney cancer cell lines using
various cell viability assays and compare its efficacy with the standard chemotherapeutic drug, Vinblastine.
Objective: To determine the dose-dependent cytotoxicity of Encequidar and assess its potential as a novel
treatment option for transitional cell carcinoma (TCC). Research: Encequidar was tested at concentrations of 1
μM, 5 μM, and 10 μM using MTT, CellTiter-Glo, Alamar Blue, SRB, and LDH Cytotoxicity assays. The results
were analyzed in terms of cell viability, proliferation, and cytotoxicity to determine the therapeutic efficacy of
Encequidar relative to Vinblastine. Conclusion: Encequidar showed significant cytotoxic effects at higher
concentrations, with cell viability reduced to less than 40% in most assays at 10 μM concentration. The findings
indicate that Encequidar may serve as a promising candidate for the treatment of TCC, warranting further
preclinical and clinical investigations.

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