World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF PREGABLIN AND 4-ISOBUTYLPYRROLIDIN-2-ONE USING ANIMAL MODELS

Dr. Syed Ahmed Hussain*, Faheem Unnisa*, Arshiya Tarannum, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima and Nazneen

ABSTRACT

Aim: To evaluate the cytotoxic effects of Gemcitabine elaidate on Transitional cell carcinoma cell lines using a
series of viability assays. Objective: This study aims to investigate the efficacy of Gemcitabine elaidate as a
therapeutic agent for Transitional cell carcinoma, comparing its activity to the standard treatment, Vinblastine. The
investigation includes multiple cell viability and cytotoxicity assays to measure cell response and to determine
Gemcitabine elaidate's potential as a combination therapy agent. Research: Transitional cell carcinoma cell lines
were treated with various concentrations of Gemcitabine elaidate (1 μM, 5 μM, and 10 μM). Cell viability was
measured using MTT, CellTiter-Glo, Alamar Blue, SRB, and LDH Cytotoxicity Assays. The results showed a
concentration-dependent decrease in cell viability, with higher concentrations leading to more significant cytotoxic
effects. Each assay provided a comprehensive evaluation of the molecule’s impact on cellular metabolic activity,
total protein content, ATP levels, and cell membrane integrity. Conclusion: The results indicate that Gemcitabine
elaidate demonstrates notable cytotoxicity in Transitional cell carcinoma cell lines, particularly at higher
concentrations, suggesting its potential utility in combination therapies for treating Transitional cell carcinoma.

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