World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF TRANSITIONAL CELL CARCINOMA USING SIMILAR MOLECULE – CARBOPLATINUM

Dr. Syed Ahmed Hussain*, Faheem Unnisa*, Arshiya Tarannum, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima and Nazneen

ABSTRACT

Aim: To evaluate the cytotoxic efficacy of Carboplatinum on Transitional cell carcinoma cell lines using a variety
of cell viability assays and compare its activity to the standard chemotherapeutic drug, Vinblastine. Objective: To
investigate the dose-dependent effects of Carboplatinum on cell viability and cytotoxicity in Transitional cell
carcinoma cell lines using MTT, CellTiter-Glo Luminescent Cell Viability, Alamar Blue, Sulforhodamine B
(SRB), and LDH Cytotoxicity Assays. Research: Transitional cell carcinoma cell lines were treated with varying
concentrations of Carboplatinum (1, 5, and 10 μM). The cell viability and cytotoxicity were assessed using
multiple assays to ensure comprehensive evaluation. MTT, CellTiter-Glo, Alamar Blue, and SRB assays showed a
consistent decrease in cell viability with increasing concentrations, indicating the cytotoxic effect of
Carboplatinum. The LDH Cytotoxicity Assay revealed increased cell membrane damage at higher doses,
suggesting that the drug induces cell lysis. Conclusion: Carboplatinum demonstrated significant cytotoxic activity
against Transitional cell carcinoma cell lines, comparable to Vinblastine. These findings suggest its potential as an
alternative or adjunct chemotherapeutic agent. Further studies, including in-vivo testing and clinical trials, are
warranted to establish its safety and efficacy in treating Transitional cell carcinoma.

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