World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF SQUAMOUS CELL CARCINOMA USING SIMILAR MOLECULE – FOLFIRI

Dr. Syed Ahmed Hussain*, Arshiya Tarannum, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima, Faheem Unnisa and Nazneen

ABSTRACT

Aim: This study aims to evaluate the cytotoxic effects of Folfiri, an EGFR-targeting monoclonal antibody, in kidney cancer cell lines and to compare its efficacy with the conventional chemotherapy drug cisplatin. Objective: To assess cell viability and cytotoxicity using various assays, including MTT, CellTiter-Glo, Alamar Blue, Sulforhodamine B (SRB), and LDH assays. Research: The experiments demonstrated that Folfiri significantly reduces cell viability in a concentration-dependent manner. Results from the MTT and CellTiter-Glo assays showed that Folfiri treatment leads to decreased absorbance and luminescence, indicating reduced metabolic activity. The LDH assay further confirmed cell membrane damage, corroborating the cytotoxic effects observed. Conclusion: Folfiri exhibits potent cytotoxic activity against kidney cancer cell lines, comparable to cisplatin. These findings support the exploration of Folfiri as a promising candidate for combination therapy in SCC treatment, highlighting the potential for improved patient outcomes.

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