World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF SQUAMOUS CELL CARCINOMA USING SIMILAR MOLECULE - 3-CHLORO-4-FLUOROANILINE

Dr. Syed Ahmed Hussain*, Arshiya Tarannum, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima, Faheem Unnisa and Nazneen

ABSTRACT

Aim: This study investigates the cytotoxic effects of 3-chloro-4-fluoroaniline, an epidermal growth factor receptor (EGFR) inhibitor, on kidney cancer cell lines, comparing its effectiveness with the well-established chemotherapy drug cisplatin. Objective: To evaluate the viability of kidney cancer cells following treatment with varying concentrations of 3-chloro-4-fluoroaniline using multiple assays: MTT, CellTiter-Glo, Alamar Blue, SRB, and LDH cytotoxicity assays. Research: The study utilized five different cell viability assays to assess the cytotoxicity of 3-chloro-4-fluoroaniline at concentrations of 1 µM, 5 µM, and 10 µM against kidney cancer cell lines. The control used in all assays was cisplatin. The results showed a significant decrease in cell viability with increasing concentrations of 3-chloro-4-fluoroaniline, with the MTT assay indicating a maximum viability of 77.6% at 1 µM concentration. Conclusion: 3-Chloro-4-fluoroaniline exhibits cytotoxic effects on kidney cancer cell lines, with a dose-dependent response. These findings support further exploration of this compound as a potential therapeutic option in treating squamous cell carcinoma, especially in combination therapies with established drugs like cisplatin.

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