World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF OROPHARYNGEAL CANCER USING SIMILAR MOLECULE - 10-OXO DOCETAXEL

Umaima Batool Osmani*, Dr. Syed Ahmed Hussain, Raheem Unnisa Shaik, Maimuna Fatima, Arshiya Tarannum, Faheem Unnisa and Nazneen

ABSTRACT

Aim: This study aims to evaluate the cytotoxic effects of 10-Oxo Docetaxel on kidney cancer cell lines in comparison to the marketed drug Cetuximab. Objective: The objective is to determine the viability of kidney cancer cells treated with varying concentrations of 10-Oxo Docetaxel using different assays, thereby assessing its potential as a therapeutic agent in advanced head and neck squamous cell carcinoma (HNSCC) treatment. Research: The study employed several assays, including MTT, CellTiter-Glo, Alamar Blue, Sulforhodamine B (SRB), and LDH Cytotoxicity assays. Each assay provided insight into cell viability based on drug concentration, with results showing a significant decrease in cell viability with increasing concentrations of 10-Oxo Docetaxel. Specifically, at the highest concentration tested (10 µM), cell viability was reduced to 28.8% for MTT and 32.2% for CellTiter-Glo, indicating a substantial cytotoxic effect. Conclusion: The findings suggest that 10-Oxo Docetaxel exhibits promising cytotoxic activity against kidney cancer cell lines, comparable to Cetuximab, warranting further investigation into its mechanisms and potential clinical applications.

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