World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF OROPHARYNGEAL CANCER USING SIMILAR MOLECULE – CARBOPLATINUM

Umaima Batool Osmani*, Dr. Syed Ahmed Hussain, Raheem Unnisa Shaik, Maimuna Fatima, Arshiya Tarannum, Faheem Unnisa and Nazneen

ABSTRACT

Aim: The study aims to evaluate the cytotoxic effects of Carboplatinum, a platinum-based chemotherapy drug, on kidney cancer cell lines using various cell viability assays. Objective: To compare the efficacy of Carboplatinum in inhibiting cell viability at different concentrations and assess its potential as a therapeutic agent. Research: The investigation was carried out using five different assays—MTT, CellTiter-Glo, Alamar Blue, SRB, and LDH—to assess cell viability, proliferation, and cytotoxicity. Kidney cancer cell lines were treated with varying concentrations of Carboplatinum, and the assays were used to measure cell survival and cytotoxicity. Conclusion: Results indicated a dose-dependent reduction in cell viability with increased Carboplatinum concentration. Among all the assays, the SRB assay demonstrated the highest sensitivity in detecting cell viability, showing a significant decline in cell survival at the highest concentration (10 µM). The findings suggest that Carboplatinum effectively reduces cell viability in kidney cancer cell lines and has potential clinical applications as an anti-cancer agent.

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