World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF OROPHARYNGEAL CANCER USING SIMILAR MOLECULE – NIVOLUMAB

Dr. Syed Ahmed Hussain*, Umaima Batool Osmani, Raheem Unnisa Shaik, Maimuna Fatima, Arshiya Tarannum, Faheem Unnisa and Nazneen

ABSTRACT

Aim: To evaluate the efficacy of Nivolumab and similar molecules for the treatment of oropharyngeal cancer in
comparison to the marketed drug Cetuximab. Objective: The study aimed to assess the cytotoxic effects of
Nivolumab using various in vitro assays, including MTT, CellTiter-Glo, Alamar Blue, SRB, and LDH cytotoxicity
assays, to measure cell viability and cytotoxicity. Research: Oropharyngeal cancer cells were treated with varying
concentrations of Nivolumab, and cell viability was measured using multiple assay techniques. Nivolumab showed
a dose-dependent decrease in cell viability, demonstrating its potential as an effective therapeutic agent. The results
were consistent across the assays, indicating reliable cytotoxicity at higher concentrations. Conclusion: Nivolumab
exhibited substantial cytotoxic effects in oropharyngeal cancer cells, reducing cell viability more effectively than
Cetuximab. These findings suggest that Nivolumab can be considered a promising therapeutic option for the
treatment of oropharyngeal cancer. Further studies are recommended to validate these results in clinical settings.

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