World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF TRANSITIONAL CELL CARCINOMA USING SIMILAR MOLECULE - FLAVOPIRIDOL HYDROCHLORIDE HYDRATE

Dr. Syed Ahmed Hussain*, Raheem Unnisa Shaik, Maimuna Fatima, Umaima Batool Osmani, Arshiya Tarannum, Faheem Unnisa and Nazneen

ABSTRACT

Aim: The study aimed to investigate the potential efficacy of Flavopiridol hydrochloride hydrate, a novel
compound targeting cell cycle machinery, in comparison to the marketed drug Pembrolizumab in treating Non-
Small Cell Lung Cancer (NSCLC). Objective: The primary objective was to evaluate the cytotoxic effects of
Flavopiridol across various concentrations using five distinct cell viability assays: MTT, CellTiter-Glo, Alamar
Blue, SRB, and LDH Cytotoxicity Assay. Research: The research utilized several assays to assess cell viability in
kidney cancer cell lines treated with Flavopiridol. Results indicated varying degrees of cytotoxicity at different
concentrations, with Flavopiridol showing significant reduction in cell viability compared to the control,
Pembrolizumab. The MTT assay revealed a cell viability of 29.8% at the highest concentration of Flavopiridol,
while the LDH assay indicated 28% cell viability. Conclusion: Flavopiridol hydrochloride hydrate demonstrates
potential as an effective treatment for NSCLC, particularly when used in conjunction with existing therapies.
Further studies are warranted to explore its mechanisms and efficacy in clinical settings.

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