World Journal of
Pharmaceutical and Life Sciences

IN-VITRO EVALUATION OF CELL VIABILITY STUDIES OF TRANSITIONAL CELL CARCINOMA USING SIMILAR MOLECULE - SALINOMYCIN SODIUM SALT

Dr. Syed Ahmed Hussain*, Raheem Unnisa Shaik, Maimuna Fatima, Umaima Batool Osmani, Arshiya Tarannum, Faheem Unnisa and Nazneen

ABSTRACT

Aim: The aim of this study is to evaluate the potential of salinomycin sodium salt as a treatment for Non-Small Cell Lung Cancer (NSCLC) in comparison to the marketed drug Pembrolizumab (Keytruda). Objective: This research seeks to explore the effectiveness of salinomycin sodium salt, a molecule targeting the WNT pathway, in enhancing cell viability and cytotoxicity in kidney cancer cell lines. Research: Utilizing various assays, including MTT, CellTiter-Glo, Alamar Blue, Sulforhodamine B (SRB), and LDH cytotoxicity, we measured cell viability and proliferation in response to different concentrations of salinomycin sodium salt and Pembrolizumab. The assays demonstrated that both compounds exhibit a dose-dependent effect on cell viability, with Pembrolizumab maintaining higher viability percentages across treatments. Conclusion: The findings suggest that while salinomycin sodium salt shows promise in reducing cell viability, Pembrolizumab remains a more effective treatment in the evaluated concentrations. Future research should focus on further elucidating the mechanisms of action of salinomycin sodium salt and exploring combination therapies to improve NSCLC treatment outcomes.

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