World Journal of
Pharmaceutical and Life Sciences

INVITRO EVALUATION OF ISONIAZID AND ITS DERIVATIVE (ISONIAZID SODIUM METHANESULFONATE) FOR THE TREATMENT OF TUBERCULOSIS

Dr. Syed Ahmed Hussain* and Saniya Begum

ABSTRACT

Aim: The aim of this study is to evaluate the biological effects of Isoniazid sodium methanesulfonate on TB cells using various assays to determine its influence on cell viability, tubulogenesis, and protein expression. Objective: To assess the cytotoxic potential of Isoniazid sodium methanesulfonate in HT-29 and HUVEC cell lines using MTT, Tubulogenesis, Indirect Immunofluorescence, and Western Blot assays and to compare its activity with a control and standard group. Research: The study utilized a series of in vitro assays, including the MTT assay to evaluate cell viability, the Tubulogenesis assay to measure its effect on tube formation in endothelial cells, the Indirect Immunofluorescence assay to determine the expression levels of NF-κB and VEGF-A in treated cells, and Western Blot analysis to validate protein expression. The results showed a significant decrease in cell viability and tube formation in the treated groups, with a corresponding decrease in NF-κB and VEGF-A expression levels. Conclusion: Isoniazid sodium methanesulfonate demonstrated significant cytotoxic and anti-angiogenic activity in TB cells. The findings suggest that it may serve as a potential therapeutic agent for TB treatment due to its ability to suppress cell proliferation, inhibit angiogenesis, and reduce the expression of key proteins involved in TB progression.

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