World Journal of
Pharmaceutical and Life Sciences

DETECTION OF HEAT SHOCK PROTEIN 70 IN GASTROINTESTINAL ORGANS IN EXPERIMENTAL FLUOROSIS BY ELISA

Shashi A.* and Sukhmanjeet Kaur

ABSTRACT

Background: The essential role that inducible heat shock protein 70 plays in sustaining gastrointestinal deficits may be explained by its multiple functions as an immunomodulatory and cytoprotective protein. The present study used ELISA to detect heat shock protein 70 in the gastrointestinal organs in fluorosis and therapeutic impact of Boerhaavia diffusa L. Materials and Methods: Thirty six rats were divided randomly into six groups with six rats in each group. Group-1 (C1) received deionized water while Group-2 was administered with 600 mg NaF/kg b.w./day for 40 days via oral gavage. Group- 3 (C2) and Group- 4 (C4) were administered 250 mg and 500 mg/kg b.w./day of leaf extract of Boerhaavia diffusa L. for 20 days respectively. While Group-5 and Group-6 received 600 mg of sodium fluoride for 40 days were post-treated with 250 and 500 mg/kg b.w./day of Boerhaavia diffusa L. for 20 days. The rats were sacrificed and samples from stomach, small intestine and large intestine were taken out. HSP 70 protein expression was then analyzed using ELISA. Results: The results revealed that in stomach, small intestine and large intestine the rats exposed to 600 mg/kg b.w./day of NaF had higher mean levels of HSP 70 as compared to control group. After post-treatment with Boerhaavia diffusa L. for 20 days, the tissues of fluoridated rats had comparatively lower mean levels of HSP 70. Conclusion: These findings demonstrated a significant relationship between the level of fluoride in gastrointestinal tissue and HSP 70 in rats. Furthermore, this research provides evidence supporting the reduction of fluoride toxicity and promotes improved well-being for animals.

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