World Journal of
Pharmaceutical and Life Sciences

DEVELOPMENT AND VALIDATION OF RP-HPLC ANALYTICAL METHOD FOR THE QUANTITATIVE ESTIMATION OF CILNIDIPINE IN PURE FORM AND MARKETED FORMULATION

K. Pavan Kalyan Goud*, G. Priyanka, A. Rajamani and A. Yasodha

ABSTRACT

A simple, rapid, specific and accurate reverse phase high performance liquid chromatographic method has been developed for the validated of Cilnidipine in bulk as well as in marketed pharmaceutical dosage form. This separation was performed on a Symmetry ODS (C18) RP Column, 250 mm x 4.6 mm, 5μm column with ACN : Methanol: 0.1% OPA in the ratio of 60:30:10 as mobile phase at a flow rate of 1.0 mL min−1 with UV detection at 235 nm; the constant column temperature was Ambient. The run time under these chromatographic conditions was less than 6.0 min. The retention time of Cilnidipine was found to be 2.570min. The calibration plot was linear over the concentration range of 6–14 μg mL−1 with limits of detection and quantification values of 0.8 and 0.24ng mL−1 respectively. The mean % assay of marketed formulation was found to be 99.79%, and % recovery was observed in the range of 98-102%. Relative standard deviation for the precision study was found <2%.The developed method is simple, precise, specific, accurate and rapid, making it suitable for estimation of Cilnidipine in bulk and marketed pharmaceutical dosage form dosage form.

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