World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF IN-VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT 0F SYZYGIUM OLEOSUM IN WISTAR RATS

Ravi Singh, Prajakta Maskawade*, Shreena Biyani and Dr. Karunakar Shukla

ABSTRACT

Natural products are always helpful in the maintenance of life and good health. Syzygium oleosum (S. oleosum). is a very large evergreen tropical tree belonging to the family Myrtaceae. The plant is also mentioned in literature as Jamun, synonym as black plum. The native home of the Syzygium is India and East Indies. This plant is also found in other countries like Thailand, Philippines, Madagascar. Extensive work was carried out on plant of S. oleosum for their pharmacological properties. The medicinal value is due to presence of malic acid, oxalic acid, gallic acid, tannins. Various works on tannin, flavonoids essential oil and betulinic acid was reported to have diverse pharmacological activities like gastroprotective, antiulcerogenic, antibacterial, anti-infective, antimalarial. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Antioxidant activity was studied through DPPH assay, ABTS+ radical scavenging assay method using ascorbic acid as standards. The present study is aimed to evaluate the anti-inflammatory activity of S. oleosum on histamine induced rat paw edema method in rats as for controlling inflammatory disorders. Acute toxicity of the extract (2000 mg/kg) was examined in wistar rats for 14 days. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, glycosides, alkaloids. The total phenolics content of S. oleosum extract was (71.94 mg/g/100mg), followed by flavonoids (1.81 mg/g /100mg) respectively. The activities of ethanolic bark extract against DPPH assay, ABTS+ radical scavenging assay, were concentration dependent. Ethanolic extract up to 2000 mg/kg did not produce any toxic effects. The ethanolic extract of S. oleosum (200 and 400 mg/kg) inhibited the inflammation induced by histamine in rats in a dose dependent manner. The ethanolic extract of S. oleosum possesses a strong anti-inflammatory activity and may be considered an interesting source of effective anti-inflammatory compounds.

[Full Text Article]   [Download Certificate]