World Journal of
Pharmaceutical and Life Sciences

EFFECTIVENESS TEST OF SECONDARY METABOLITES TRICHODERMA SP. AND PSEUDOMONAS FLUORESCENS AS THE BIOLOGICAL CONTROL AGENCY OF THE GANODERMA BONINENSE PATHOGENS IN THE LABORATORY

Muklasin, Syamsafitri* and Dan Yenni Asbur

ABSTRACT

Ganoderma boninense is a pathogen that causes stem rot in oil palm plants. This pathogen is considered important because it can cause losses of around 50-80% per ha, can attack and spread in plant tissue from seedbeds to mature plants till plants rot, die and eventually fall. In addition, the disease has a wide host range and special structure so that it is able to survive and infect target plants. Various efforts have been made to control stem rot disease in oil palm plants but have not been completely successful. This study aims to test the effectiveness of secondary metabolites produced by Trichoderma sp. and Pseudomonas fluorescens singly or in combination as the biological control agencies pathogen G. boninense in the laboratory of the BBPPTP Medan. A completely randomized design was used to test four treatments, namely secondary metabolites of Trichoderma sp., P. fluorescens, Trichoderma sp. + P. fluorescens, and control were repeated ten times. The observation parameter was the percentage of inhibition of G. boninense colony length on PDA culture media. The research results showed that the secondary metabolites of Trichoderma sp., P. fluorescens, singly or in combination, were able to inhibit the colony length of the pathogen G. boninense which causes stem rot disease in the laboratory. The percentage of inhibition of colony length by secondary metabolites of Trichoderma sp., P. fluorescens or a combination of both respectively was 11.50%; 22.13% and 17.88%.

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