World Journal of
Pharmaceutical and Life Sciences

EFFECTIVENESS TEST OF THE TRICHODERMA SP. AND PSEUDOMONAS FLUORESCENS SECONDARY METABOLITES AS BIOLOGICAL CONTROL AGENCIES OF THE ONCOBASIDIUM THEOBROMA PATHOGENS IN THE LABORATORY

Christina Oktora Matondang, Syamsafitri*, Nurhayati and Yenni Asbur

ABSTRACT

Oncobasidium theobroma is a pathogen that causes Vascular Streak Dieback in cocoa plants. Vascular streak dieback or VSD is an important disease of cocoa and can makes plants become bare and decrease fruit production. Many attemps have been made to control VSD disease on cocoa but have not been succesfull yet. This study aimed to examine the potency of secondary metabolite produced by Trichoderma sp. and Pseudomonas fluorescens solely or in combination as biological control agencies of the pathogen O. theobroma in the laboratory at Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan (BBPPTP) Medan. A completely randomized design was used yo assessed four treatments i.e Trichoderma sp., P. fluorescens, Trichoderma sp. + P. fluorescens secondary metabolites and control which was repeated six times. The observation parameter is the length of the colony of pathogen O. theobroma on the culture medium or PDA. The results showed that the secondary metabolites of Trichoderma sp., P. fluorescens, Trichoderma sp. + P. fluorescens secondary metabolites were able to suppress or inhibit the colony length of the pathogen O. theobroma which causes VSD disease in the laboratory. The percentage of inhibition of colony length by secondary metabolites of Trichoderma sp., P. fluorescens or a combination of both respectively was 92.55%; 72.05% and 63.6%.

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