World Journal of
Pharmaceutical and Life Sciences

METHOD DEVELOPMENT AND SIMULTANEOUS ESTIMATION OF ACETAMINOPHEN AND IT'S RELATED SUBSTANCE BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY (LC-MS)

S. Karthikeyan, M. Bakyalakshmi*, P. D. Gokulan and K. L. Senthil Kumar

ABSTRACT

Development and Analysis of drugs involve the most stimulating work in the medicinal field in that way, in that
way, there emergence of new drug in day to day. Acetaminophen is a common Over the counter drug used by
worldwide as the use of this drug increased globally requirement for detecting its impurities is mandatory and its
determination of related substance were evaluated by using Liquid Chromatography Mass spectrometry, this
method has emerged as a capable and multipurpose tool for analysing various types of drug product and its
impurities. It has the capability of detecting small molecule to high molecule with high sensitivity. The optimized
method was developed for estimation of related substance of acetaminophen using a gradient program mobile
phase of formic acid and acetonitrile with 0.6 ml/min flow at 210 and 254 nm wavelength with optimized mass
condition. In this paper, briefly discussed about method development for determination of related substance of
acetaminophen along with its active pharmaceutical ingredient in a finished marketed product.

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