World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

SCREENING & OPTIMIZATION OF PHYSIOLOGICAL PARAMETERS FOR PRODUCTION OF LIPASE USING DE-OILED SEED CAKE AS A SUBSTRATE.

Asmita R Shringeri and G. R. Naik*

ABSTRACT

The present work was conducted in the Laboratory of Biotechnology, Department of Biotechnology, Gulbarga University Kalaburagi, Karnataka, with the objective of process optimization studies on physiological parameters in the production process namely pH and temperature. It deals with the screening of microorganisms with different substrates- pongamia seed cake, jatropha seed cake, simarouba seed cake. The optimum parameters for maximum lipase production were carried out for four days of incubation, inoculums size 0.25ml to 1ml, pH 5.0-10.0, moisture content and 37°C-60oC incubation temperature. Our results suggested that solid state fermentation process is a tool to utilize the low-cost substrate like simarouba seed cake, jatropha seed cake, pongamia seed cake for the production of industrially important enzyme such as lipase.

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