World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

INVITRO EVALUATION OF METHOTREXATE AND ITS DERIVATIVE (DL-METHOTREXATE (+)- AMETHOPTERIN) FOR THE TREATMENT OF INTRAOCULAR (EYE) MELANOMA

Dr. Syed Ahmed Hussain*, Juveria Gaffar, Farahanaaz Begum, Shaik Nazma Sultana and Sara Umm E. Hani

ABSTRACT

This research paper presents a study on the effects of DL-Methotrexate(+)-Amethopterin on cell viability and functionality. DL-Methotrexate(+)-Amethopterin is a medication used for its anti-inflammatory and anti-proliferative properties, similar to Methotrexate. In this study, we employed multiple assays to investigate the impact of DL-Methotrexate(+)-Amethopterin on cell behavior. The assays included MTT Assay, Tubulogenesis Assay, Indirect Immunofluorescence Assay, and Western Blot Analysis. The results of these assays provide insights into the influence of DL-Methotrexate(+)-Amethopterin on cell viability and its potential implications in the context of therapeutic applications.

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