World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

INVITRO EVALUATION OF METHOTREXATE AND ITS DERIVATIVE (METHOTREXATE 5-METHYL ESTER) FOR THE TREATMENT OF INTRAOCULAR (EYE) MELANOMA

Juveria Gaffar*, Dr. Syed Ahmed Hussain, Farahanaaz Begum, Shaik Nazma Sultana and Sara Umm E. Hani

ABSTRACT

This research paper presents a study on the effects of Methotrexate 5-Methyl Ester on cell viability and functionality. Methotrexate 5-Methyl Ester is a derivative of Methotrexate, a well-known medication used for its anti-inflammatory and anti-proliferative properties. In this study, we conducted multiple assays to investigate the impact of Methotrexate 5-Methyl Ester on cell behavior. The assays included MTT Assay, Tubulogenesis Assay, Indirect Immunofluorescence Assay, and Western Blot Analysis. The results of these assays provide insights into the influence of Methotrexate 5-Methyl Ester on cell viability and its potential implications in the context of therapeutic applications.

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