World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

INVITRO EVALUATION OF METHOTREXATE AND ITS DERIVATIVE (METHOTREXATE DISODIUM) FOR THE TREATMENT OF INTRAOCULAR (EYE) MELANOMA

Dr. Syed Ahmed Hussain*, Juveria Gaffar, Farahanaaz Begum, Shaik Nazma Sultana and Sara Umm E. Hani

ABSTRACT

This research paper presents the results of an experimental study that explores the effects of Methotrexate Disodium on various cellular assays, including MTT, Tubulogenesis, Indirect Immunofluorescence, and Western Blot Analysis. The study comprises four treatment groups, including normal cells, a control cell line, a group treated with standard Methotrexate, and a group treated with Methotrexate Disodium. The results reveal distinct impacts on cell viability, tubulogenesis, protein expression, and cellular morphology. These findings contribute to the understanding of Methotrexate Disodium's potential as a therapeutic agent and its implications for future research and clinical applications.

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