World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF IN VITRO CYTOTOXIC ACTIVITY OF ETHANOLIC EXTRACT OF ROOT OF IPOMOEA BATATAS (L) (EERIB) AGAINST THE HUMAN COLORECTAL ADENOCARCINOMA HT-29 CELL LINE

Dr. Mukuntha Kumar. N*, Asish Bhaumik, Shubhankar Saha

ABSTRACT

HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer. In addition to being a xenograft tumor model for colorectal cancer, the HT-29 cell line is also used as an in-vitro model to study absorption, transport, and secretion by intestinal cells. Under standard culture conditions, these cells grow as a nonpolarized, undifferentiated multilayer. Altering culture conditions or treating the cells with various inducers, however, results in a differentiated and polarized morphology, characterized by the redistribution of membrane antigens and development of an apical brush-border membrane. The main aim and objective of the present research work was the evaluation of in vitro Cytotoxic activity of ethanolic extract of root of Ipomoea batatas (L) (EERIB) against the human colorectal adenocarcinoma HT-29 cell line. The in vitro cytotoxic activity was carried out by SRB assay. The results obtained from the in-vitro studies performed by SRB assay by using human colorectal adenocarcinoma HT-29 cell line displayed that the EERIB possessed a very good cytotoxic activity. From the present studied it had been concluded that EERIB was exhibiting the potential cytotoxic action on HT-29 cell line which was proved by using standard drug 5-FU and it was found that EERIB showed the highest 90.95% growth inhibition at 10 ?g (IC50 = 2.7 ?g/ml. The IC50 value of standard drug 5-FU was found to be 1.3 ?g/ml with 95.97% growth inhibition at concentration 75 ?g/ml.

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