World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

METHOD DEVELOPMENT VALIDATION OF HPLC IN ANALYTICAL CHEMISTRY

Dr. V. Sreejanardhanan*

ABSTRACT

The development of novel techniques for non-pharmacopoeial products is crucial as it can significantly reduce the time and cost required to achieve better and more distinctive outcomes. In order to guarantee the quality, dependability, and consistency of these newly developed methods, it is essential to validate them in accordance with the International Conference on Harmonization (ICH) guidelines. Different types of validation processes, including Prospective, Concurrent, Retrospective, and Revalidation, should be employed to ensure reliability. This study also addresses the prioritization of validation, protocols, and the use of High-Performance Liquid Chromatography (HPLC) instruments. Additionally, the advantages and limitations of HPLC are emphasized.

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