World Journal of
Pharmaceutical and Life Sciences

SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND ZIDOVUDINE USING Π- ACCEPTORS AS ANALYTICAL REAGENTS: A SPECTROPHOTOMETRIC STUDY

Ravi M., Veeraiah T.* and Venkata Ramana Reddy Ch

ABSTRACT

Based on a new concept of AUC (Area Under Curve), two new sensitive and precise spectrophotometric methods have been proposed and developed for the simultaneous estimation of Lamivudine and Zidovudine in pure mixture and in pharmaceutical binary dosage forms using analytical reagents, DDQ (2,3-Dichloro-5,6-dicyano-1,4- benzoquinone) and p-CA (p-Chloranilic acid: 2,5-Dichloro-3,6-dihydroxy-1,4-benzoquinone). Method 1 involves the use of DDQ as analytical reagent and the AUC between 390 nm and 690 nm for DDQ was used for determination. Method 2 involves the use of p-CA as an analytical reagent and the AUC between 400 nm and 700 nm for p-CA was used for determination. The methods developed and construction of calibration curves using two analytical reagents viz., DDQ and p-CA are described. Optical and analytical parameters for the individual and simultaneous determination of Lamivudine and Zidovudine using AUC are tabulated. The methods have been validated and compared with HPLC methods in terms of standard deviation, t-tests and F-tests.

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