World Journal of
Pharmaceutical and Life Sciences

PHYTOCHEMICAL ANALYSIS AND ANTI-INFLAMMATORY ACTIVITY OF MEDICINAL PLANT AQUEOUS AND ETHANOLIC EXTRACT OF BOERHAAVIA DIFFUSA LEAVES EXTRACTS

K. Padmalochana* and K. Sabuna

ABSTRACT

In this study aqueous and ethanol extract of Boerhaavia diffua leaves was qualitatively assessed the presence of phytochemicals for its anti-inflammatory activity by in-vitro methods. Phytochemical analysis of aqueous and ethanol extract of leaves revealed the presence of tannins, flavonoids, terpenoids, phenols and alkaloids. Invitro anti- inflammatory activity was evaluated against albumin denaturation, proteinase activity, membrane stabilization, and lipoxygenase activity at different concentrations of aqueous and ethanol extract of leaves. The results showed that IC50 values for aqueous and ethanol extractat a concentration of 325.71±2.03 and 222.4±1.43?g/ml, respectively highly protects the heat induced protein denaturation. At the concentration of 394.31±1.93 and 279.43±2.77?g/ml, aqueous and ethanol extract showed significant 50% (IC50) of proteinase inhibitory action. Heat induced haemolysis of erythrocyte was significantly 50% of inhibited atthe concentration of 300.47±2.57 and 238.97±2.03 ?g/ml for aqueous and ethanol extract, respectively. Hypotonicity induced haemolysis and lipoxygenase activity were significantly 50% inhibited at the concentrationof309.87±2.69 and300.94±1.77?g/ml, respectively by the aqueous extract. Ethanol extract showed 50% of inhibition of hypotonicity induced haemolysis and lipoxygenase activity was found to be 205.94±2.07 and259.34±2.33?g/ml, respectively. The results obtained in the present study indicate that ethanol extracts of B. diffusa leaves can be a potential source of anti-inflammatory agents compared than aqueous extract.

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